polysomal fractions Search Results


polysomal ribosomes  (Thermo Fisher)
99
Thermo Fisher polysomal ribosomes
(A) Schematic of the experimental system to measure the architecture of active protein synthesis <t>(polysomal</t> ribosomes) from the ex vivo neocortex across embryonic (E12.5, E14, E15.5, E17) and early postnatal (P0) neurogenesis. See text for details. (B) MS analysis of neocortical polysomal complexes across development. Scatter plots compare early neurogenesis E12.5 vs. each subsequent stage, demonstrating the enrichment of Ebp1 (red arrow) among ribosomal proteins (RPs) of the large (Rpl, blue) and small (Rps, yellow) subunits, in contrast to other translation-associated proteins (black). (C) Stoichiometry cluster heat maps quantifying the differential enrichment of each RP (Rpl, blue; Rps, yellow), translation-associated protein (black), and Ebp1 (red arrow) per developmental stage. Expression of adjacent proteins on the x-axis is shown as higher (orange), lower (purple), or similar (black) relative to each protein on the y-axis. Legend and histogram at top left for each stage. See also - .
Polysomal Ribosomes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fraction polys  (Cytos Inc)
90
Cytos Inc fraction polys
(A) Schematic of the experimental system to measure the architecture of active protein synthesis <t>(polysomal</t> ribosomes) from the ex vivo neocortex across embryonic (E12.5, E14, E15.5, E17) and early postnatal (P0) neurogenesis. See text for details. (B) MS analysis of neocortical polysomal complexes across development. Scatter plots compare early neurogenesis E12.5 vs. each subsequent stage, demonstrating the enrichment of Ebp1 (red arrow) among ribosomal proteins (RPs) of the large (Rpl, blue) and small (Rps, yellow) subunits, in contrast to other translation-associated proteins (black). (C) Stoichiometry cluster heat maps quantifying the differential enrichment of each RP (Rpl, blue; Rps, yellow), translation-associated protein (black), and Ebp1 (red arrow) per developmental stage. Expression of adjacent proteins on the x-axis is shown as higher (orange), lower (purple), or similar (black) relative to each protein on the y-axis. Legend and histogram at top left for each stage. See also - .
Fraction Polys, supplied by Cytos Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
fraction polys - by Bioz Stars, 2026-04
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mirna isolation kit  (Qiagen)
90
Qiagen mirna isolation kit
(A) Schematic of the experimental system to measure the architecture of active protein synthesis <t>(polysomal</t> ribosomes) from the ex vivo neocortex across embryonic (E12.5, E14, E15.5, E17) and early postnatal (P0) neurogenesis. See text for details. (B) MS analysis of neocortical polysomal complexes across development. Scatter plots compare early neurogenesis E12.5 vs. each subsequent stage, demonstrating the enrichment of Ebp1 (red arrow) among ribosomal proteins (RPs) of the large (Rpl, blue) and small (Rps, yellow) subunits, in contrast to other translation-associated proteins (black). (C) Stoichiometry cluster heat maps quantifying the differential enrichment of each RP (Rpl, blue; Rps, yellow), translation-associated protein (black), and Ebp1 (red arrow) per developmental stage. Expression of adjacent proteins on the x-axis is shown as higher (orange), lower (purple), or similar (black) relative to each protein on the y-axis. Legend and histogram at top left for each stage. See also - .
Mirna Isolation Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mirna isolation kit/product/Qiagen
Average 90 stars, based on 1 article reviews
mirna isolation kit - by Bioz Stars, 2026-04
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trizol ls  (Thermo Fisher)
97
Thermo Fisher trizol ls
(A) Schematic of the experimental system to measure the architecture of active protein synthesis <t>(polysomal</t> ribosomes) from the ex vivo neocortex across embryonic (E12.5, E14, E15.5, E17) and early postnatal (P0) neurogenesis. See text for details. (B) MS analysis of neocortical polysomal complexes across development. Scatter plots compare early neurogenesis E12.5 vs. each subsequent stage, demonstrating the enrichment of Ebp1 (red arrow) among ribosomal proteins (RPs) of the large (Rpl, blue) and small (Rps, yellow) subunits, in contrast to other translation-associated proteins (black). (C) Stoichiometry cluster heat maps quantifying the differential enrichment of each RP (Rpl, blue; Rps, yellow), translation-associated protein (black), and Ebp1 (red arrow) per developmental stage. Expression of adjacent proteins on the x-axis is shown as higher (orange), lower (purple), or similar (black) relative to each protein on the y-axis. Legend and histogram at top left for each stage. See also - .
Trizol Ls, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/trizol ls/product/Thermo Fisher
Average 97 stars, based on 1 article reviews
trizol ls - by Bioz Stars, 2026-04
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trizol reagent  (Thermo Fisher)
90
Thermo Fisher trizol reagent
(A) Schematic of the experimental system to measure the architecture of active protein synthesis <t>(polysomal</t> ribosomes) from the ex vivo neocortex across embryonic (E12.5, E14, E15.5, E17) and early postnatal (P0) neurogenesis. See text for details. (B) MS analysis of neocortical polysomal complexes across development. Scatter plots compare early neurogenesis E12.5 vs. each subsequent stage, demonstrating the enrichment of Ebp1 (red arrow) among ribosomal proteins (RPs) of the large (Rpl, blue) and small (Rps, yellow) subunits, in contrast to other translation-associated proteins (black). (C) Stoichiometry cluster heat maps quantifying the differential enrichment of each RP (Rpl, blue; Rps, yellow), translation-associated protein (black), and Ebp1 (red arrow) per developmental stage. Expression of adjacent proteins on the x-axis is shown as higher (orange), lower (purple), or similar (black) relative to each protein on the y-axis. Legend and histogram at top left for each stage. See also - .
Trizol Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/trizol reagent/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
trizol reagent - by Bioz Stars, 2026-04
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100 kda amicon  (Millipore)
90
Millipore 100 kda amicon
(A) Schematic of the experimental system to measure the architecture of active protein synthesis <t>(polysomal</t> ribosomes) from the ex vivo neocortex across embryonic (E12.5, E14, E15.5, E17) and early postnatal (P0) neurogenesis. See text for details. (B) MS analysis of neocortical polysomal complexes across development. Scatter plots compare early neurogenesis E12.5 vs. each subsequent stage, demonstrating the enrichment of Ebp1 (red arrow) among ribosomal proteins (RPs) of the large (Rpl, blue) and small (Rps, yellow) subunits, in contrast to other translation-associated proteins (black). (C) Stoichiometry cluster heat maps quantifying the differential enrichment of each RP (Rpl, blue; Rps, yellow), translation-associated protein (black), and Ebp1 (red arrow) per developmental stage. Expression of adjacent proteins on the x-axis is shown as higher (orange), lower (purple), or similar (black) relative to each protein on the y-axis. Legend and histogram at top left for each stage. See also - .
100 Kda Amicon, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/100 kda amicon/product/Millipore
Average 90 stars, based on 1 article reviews
100 kda amicon - by Bioz Stars, 2026-04
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rneasy mini kit  (Qiagen)
96
Qiagen rneasy mini kit
(A) Schematic of the experimental system to measure the architecture of active protein synthesis <t>(polysomal</t> ribosomes) from the ex vivo neocortex across embryonic (E12.5, E14, E15.5, E17) and early postnatal (P0) neurogenesis. See text for details. (B) MS analysis of neocortical polysomal complexes across development. Scatter plots compare early neurogenesis E12.5 vs. each subsequent stage, demonstrating the enrichment of Ebp1 (red arrow) among ribosomal proteins (RPs) of the large (Rpl, blue) and small (Rps, yellow) subunits, in contrast to other translation-associated proteins (black). (C) Stoichiometry cluster heat maps quantifying the differential enrichment of each RP (Rpl, blue; Rps, yellow), translation-associated protein (black), and Ebp1 (red arrow) per developmental stage. Expression of adjacent proteins on the x-axis is shown as higher (orange), lower (purple), or similar (black) relative to each protein on the y-axis. Legend and histogram at top left for each stage. See also - .
Rneasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rneasy mini kit/product/Qiagen
Average 96 stars, based on 1 article reviews
rneasy mini kit - by Bioz Stars, 2026-04
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allprep rna protein kit  (Qiagen)
96
Qiagen allprep rna protein kit
(A) Schematic of the experimental system to measure the architecture of active protein synthesis <t>(polysomal</t> ribosomes) from the ex vivo neocortex across embryonic (E12.5, E14, E15.5, E17) and early postnatal (P0) neurogenesis. See text for details. (B) MS analysis of neocortical polysomal complexes across development. Scatter plots compare early neurogenesis E12.5 vs. each subsequent stage, demonstrating the enrichment of Ebp1 (red arrow) among ribosomal proteins (RPs) of the large (Rpl, blue) and small (Rps, yellow) subunits, in contrast to other translation-associated proteins (black). (C) Stoichiometry cluster heat maps quantifying the differential enrichment of each RP (Rpl, blue; Rps, yellow), translation-associated protein (black), and Ebp1 (red arrow) per developmental stage. Expression of adjacent proteins on the x-axis is shown as higher (orange), lower (purple), or similar (black) relative to each protein on the y-axis. Legend and histogram at top left for each stage. See also - .
Allprep Rna Protein Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/allprep rna protein kit/product/Qiagen
Average 96 stars, based on 1 article reviews
allprep rna protein kit - by Bioz Stars, 2026-04
96/100 stars
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trizol  (Millipore)
90
Millipore trizol
(A) Schematic of the experimental system to measure the architecture of active protein synthesis <t>(polysomal</t> ribosomes) from the ex vivo neocortex across embryonic (E12.5, E14, E15.5, E17) and early postnatal (P0) neurogenesis. See text for details. (B) MS analysis of neocortical polysomal complexes across development. Scatter plots compare early neurogenesis E12.5 vs. each subsequent stage, demonstrating the enrichment of Ebp1 (red arrow) among ribosomal proteins (RPs) of the large (Rpl, blue) and small (Rps, yellow) subunits, in contrast to other translation-associated proteins (black). (C) Stoichiometry cluster heat maps quantifying the differential enrichment of each RP (Rpl, blue; Rps, yellow), translation-associated protein (black), and Ebp1 (red arrow) per developmental stage. Expression of adjacent proteins on the x-axis is shown as higher (orange), lower (purple), or similar (black) relative to each protein on the y-axis. Legend and histogram at top left for each stage. See also - .
Trizol, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/trizol/product/Millipore
Average 90 stars, based on 1 article reviews
trizol - by Bioz Stars, 2026-04
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2x protease inhibitor cocktail  (Millipore)
90
Millipore 2x protease inhibitor cocktail
(A) Schematic of the experimental system to measure the architecture of active protein synthesis <t>(polysomal</t> ribosomes) from the ex vivo neocortex across embryonic (E12.5, E14, E15.5, E17) and early postnatal (P0) neurogenesis. See text for details. (B) MS analysis of neocortical polysomal complexes across development. Scatter plots compare early neurogenesis E12.5 vs. each subsequent stage, demonstrating the enrichment of Ebp1 (red arrow) among ribosomal proteins (RPs) of the large (Rpl, blue) and small (Rps, yellow) subunits, in contrast to other translation-associated proteins (black). (C) Stoichiometry cluster heat maps quantifying the differential enrichment of each RP (Rpl, blue; Rps, yellow), translation-associated protein (black), and Ebp1 (red arrow) per developmental stage. Expression of adjacent proteins on the x-axis is shown as higher (orange), lower (purple), or similar (black) relative to each protein on the y-axis. Legend and histogram at top left for each stage. See also - .
2x Protease Inhibitor Cocktail, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
2x protease inhibitor cocktail - by Bioz Stars, 2026-04
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trizol-ls  (Thermo Fisher)
90
Thermo Fisher trizol-ls
(A) Schematic of the experimental system to measure the architecture of active protein synthesis <t>(polysomal</t> ribosomes) from the ex vivo neocortex across embryonic (E12.5, E14, E15.5, E17) and early postnatal (P0) neurogenesis. See text for details. (B) MS analysis of neocortical polysomal complexes across development. Scatter plots compare early neurogenesis E12.5 vs. each subsequent stage, demonstrating the enrichment of Ebp1 (red arrow) among ribosomal proteins (RPs) of the large (Rpl, blue) and small (Rps, yellow) subunits, in contrast to other translation-associated proteins (black). (C) Stoichiometry cluster heat maps quantifying the differential enrichment of each RP (Rpl, blue; Rps, yellow), translation-associated protein (black), and Ebp1 (red arrow) per developmental stage. Expression of adjacent proteins on the x-axis is shown as higher (orange), lower (purple), or similar (black) relative to each protein on the y-axis. Legend and histogram at top left for each stage. See also - .
Trizol Ls, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/trizol-ls/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
trizol-ls - by Bioz Stars, 2026-04
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rna extraction kit  (Promega)
90
Promega rna extraction kit
(A) Schematic of the experimental system to measure the architecture of active protein synthesis <t>(polysomal</t> ribosomes) from the ex vivo neocortex across embryonic (E12.5, E14, E15.5, E17) and early postnatal (P0) neurogenesis. See text for details. (B) MS analysis of neocortical polysomal complexes across development. Scatter plots compare early neurogenesis E12.5 vs. each subsequent stage, demonstrating the enrichment of Ebp1 (red arrow) among ribosomal proteins (RPs) of the large (Rpl, blue) and small (Rps, yellow) subunits, in contrast to other translation-associated proteins (black). (C) Stoichiometry cluster heat maps quantifying the differential enrichment of each RP (Rpl, blue; Rps, yellow), translation-associated protein (black), and Ebp1 (red arrow) per developmental stage. Expression of adjacent proteins on the x-axis is shown as higher (orange), lower (purple), or similar (black) relative to each protein on the y-axis. Legend and histogram at top left for each stage. See also - .
Rna Extraction Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rna extraction kit/product/Promega
Average 90 stars, based on 1 article reviews
rna extraction kit - by Bioz Stars, 2026-04
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Image Search Results


(A) Schematic of the experimental system to measure the architecture of active protein synthesis (polysomal ribosomes) from the ex vivo neocortex across embryonic (E12.5, E14, E15.5, E17) and early postnatal (P0) neurogenesis. See text for details. (B) MS analysis of neocortical polysomal complexes across development. Scatter plots compare early neurogenesis E12.5 vs. each subsequent stage, demonstrating the enrichment of Ebp1 (red arrow) among ribosomal proteins (RPs) of the large (Rpl, blue) and small (Rps, yellow) subunits, in contrast to other translation-associated proteins (black). (C) Stoichiometry cluster heat maps quantifying the differential enrichment of each RP (Rpl, blue; Rps, yellow), translation-associated protein (black), and Ebp1 (red arrow) per developmental stage. Expression of adjacent proteins on the x-axis is shown as higher (orange), lower (purple), or similar (black) relative to each protein on the y-axis. Legend and histogram at top left for each stage. See also - .

Journal: bioRxiv

Article Title: The architecture of protein synthesis in the developing neocortex at near-atomic resolution reveals Ebp1-mediated neuronal proteostasis at the 60S tunnel exit

doi: 10.1101/2020.02.08.939488

Figure Lengend Snippet: (A) Schematic of the experimental system to measure the architecture of active protein synthesis (polysomal ribosomes) from the ex vivo neocortex across embryonic (E12.5, E14, E15.5, E17) and early postnatal (P0) neurogenesis. See text for details. (B) MS analysis of neocortical polysomal complexes across development. Scatter plots compare early neurogenesis E12.5 vs. each subsequent stage, demonstrating the enrichment of Ebp1 (red arrow) among ribosomal proteins (RPs) of the large (Rpl, blue) and small (Rps, yellow) subunits, in contrast to other translation-associated proteins (black). (C) Stoichiometry cluster heat maps quantifying the differential enrichment of each RP (Rpl, blue; Rps, yellow), translation-associated protein (black), and Ebp1 (red arrow) per developmental stage. Expression of adjacent proteins on the x-axis is shown as higher (orange), lower (purple), or similar (black) relative to each protein on the y-axis. Legend and histogram at top left for each stage. See also - .

Article Snippet: Pooled 80S and polysomal ribosomes were purified ex vivo by preparative 10-50% sucrose density gradient ultracentrifugation from dissected frozen P0 mouse neocortex tissue as described above, but with the following adaptations optimizing for cryo-electron microscopy (cryo-EM).

Techniques: Ex Vivo, Expressing

Mouse neocortical lysates were subjected to preparative sucrose density gradient ultracentrifugation fractionation in biological triplicate to purify 80S and polysomal ribosome complexes at E12.5, E14, E15.5, E17, and P0. Input lysate, pooled 80S fractions, and pooled polysome fractions were analyzed by LC-MS/MS.

Journal: bioRxiv

Article Title: The architecture of protein synthesis in the developing neocortex at near-atomic resolution reveals Ebp1-mediated neuronal proteostasis at the 60S tunnel exit

doi: 10.1101/2020.02.08.939488

Figure Lengend Snippet: Mouse neocortical lysates were subjected to preparative sucrose density gradient ultracentrifugation fractionation in biological triplicate to purify 80S and polysomal ribosome complexes at E12.5, E14, E15.5, E17, and P0. Input lysate, pooled 80S fractions, and pooled polysome fractions were analyzed by LC-MS/MS.

Article Snippet: Pooled 80S and polysomal ribosomes were purified ex vivo by preparative 10-50% sucrose density gradient ultracentrifugation from dissected frozen P0 mouse neocortex tissue as described above, but with the following adaptations optimizing for cryo-electron microscopy (cryo-EM).

Techniques: Fractionation, Liquid Chromatography with Mass Spectroscopy